8/5/2023 0 Comments Imagej colony countingThis compensates for the inherent variability in plaque assays and enables reliable enumeration.Gold nanoparticles (AuNP) can increase the efficacy of radiation therapy by sensitising tumor cells to radiation damage. The BioSpot® product line conducts multiple object-oriented morphometric measurements, enabling user-defined gating to separate plaques from a complex background. Genotoxic assays use either mammalian cells (ie: mouse lymphoma assay) or microbes (ie: Ames test) and are easily imaged and counted using the BioSpot® system. Genotoxic assays are used to measure mutagenic potential by detecting forward or reverse mutations with selective media. The size and morphology of viral plaques is highly variable and notoriously difficult to detect and count. Viral plaque assays utilize either a monolayer of mammalian cells or a bacterial lawn. Localized lysis of virus-infected cells in a confluent monolayer produces a plaque that can be detected through direct staining or by staining cells in the monolayer. The BioSpot® product line enables colonies to be size-selected for enumeration, thus only counting true colonies. Mammalian colony formation assays rely on the ability to distinguish colonies from a background of single cells. Stem cell assays are used to identify multi-potent progenitor cells from bone marrow, cord blood and peripheral blood. Clonogenic assays, for example, are typically used for testing ionizing radiation and drug treatments for cancer therapeutic potential. Mammalian colony formation assays are an important tool in oncology and stem cell research, both of which rely on manual enumeration of colonies. The BioSpot® product line enables miniaturization and automation of the standard plate count, thereby producing a dramatic increase in throughput and reproducibility. Recently though, there has been an extensive effort to develop alternative (rapid) methods to replace the slow and tedious traditional methods. These include the food and dairy industries, environmental screening (water and air), personal care product testing, toxicology screening, and pharmaceutical testing.p Conventionally, these tests have been conducted manually with minimal throughput and high error rates. The ability to rapidly and reliably enumerate bacterial colonies is paramount for microbial load and bioburden testing in product safety markets. The ability to identify true colonies is critical for the analysis of both microbial and mammalian colony formation assays. This precludes the use of FACS/flow cytometry for counting colony formation assays. In contrast, fluoresence-activated cell sorting (FACS) can only enumerate single cells in suspension. By definition, colonies are clusters of cells grown on or in a solid support. The BioSpot® platform enumerates groups of cells growing as colonies and can readily distinguish colonies against a background of single cells. This provides an easily validated adaptation of compendial methods. This is naturally a tedious, error-prone, and time consuming process.īioSpot® colony counters enable automation and/or miniaturization, transforming CFU determination into a quick, easy and accurate process. Traditionally, plate counts have been determined by manually counting colonies within 60-100mm Petri dishes. Manual Microbial Colony CountingĬompendial methods for microbial load and bioburden testing specify the determination of colony forming units (CFU) per gram of sample. Compliant with 21 CFR part 11 and GLP regulations when operated with BioCompliance™.īioSpot® Colony Counters vs.Ideally suited for rapid, high-throughput operations.Proprietary illumination enables detection of colonies as small as 25 micrometers in diameter.Compatible with all plate formats from 100mm Petri dish to 384-well microplates.These include microbial load and bioburden testing, clonogenic assays, stem cell assays, Ames test, mouse lymphoma assays, viral plaque assays, and more. The BioSpot® line of instrumentation and software is highly adaptable and meets the needs of a broad range of colony counting applications. The CTL BioSpot® platform contains a wide range of automated image capture and analysis systems for microbial and mammalian colony formation assays.
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